Formatting Database for Blast+ =========================== To use any sequence by Blast/Blast+ , we need to format the fasta . Let's do it for uniprot: 1. download uniprot from the link provided ============================= ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_sprot.fasta.gz 2. issue the following command for blast+: ============================= /home/apps/ncbi/blast+/2.2.29/bin/makeblastdb -dbtype prot -in /path/to/uniprot.fasta To get blast+ commands, if you are familiar with legacy blast, use legacy_blast.pl in the following way: $ /home/apps/ncbi/blast+/2.2.29/bin/legacy_blast.pl formatdb -i test.fasta -p T --path /home/apps/ncbi/blast+/2.2.29/bin --print_only /home/apps/ncbi/blast+/2.2.29/bin/makeblastdb -dbtype prot -in test.fasta