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BLAST INSTALLATION TUTORIAL



Formatting Database for Blast+

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To use any sequence by Blast/Blast+ , we need to format the fasta . Let's do it  for uniprot:

1. download uniprot from the link provided
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ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_sprot.fasta.gz

2. issue the following command for blast+:
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/home/apps/ncbi/blast+/2.2.29/bin/makeblastdb -dbtype prot -in /path/to/uniprot.fasta


To get blast+ commands, if you are familiar with legacy blast, use legacy_blast.pl in the following way:

$ /home/apps/ncbi/blast+/2.2.29/bin/legacy_blast.pl formatdb -i test.fasta -p T --path /home/apps/ncbi/blast+/2.2.29/bin --print_only

/home/apps/ncbi/blast+/2.2.29/bin/makeblastdb -dbtype prot -in test.fasta

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